Enterovirus RNA PCR

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Enterovirus RNA PCR

Test Information:
Enteroviruses can be found in the respiratory secretions (e.g. saliva, sputum, or nasal mucus) and stool of an infected person; may cause cold-like symptoms or severe respiratory infection. Other person may become infected by direct contact with secretions from an infected person or by contact with contaminated surfaces or objects.

Specimen Container:

  • Gold Top SST Tube (with clot activator) or Red Top Tube (Plain)
  • 4N6 FLOQ Swab
  • Sterile / Plain Urine or Stool Container

Specimen Type:

  • Serum
  • Nasal/pharyngeal secretions, sputum
  • CSF

Required Volume:

  • Serum – 2 mL
  • Nasal/pharyngeal secretions, sputum – 1 mL
  • CSF – 2 mL

Patient Preparation:
No Patient Preparation

Notes:
For Nasal and Pharyngeal Secretions- Use DNA/RNA- free Dacron Swab (FloqSwab). Immerse Swab in a 2mL NSS after collection of sample.

  • Important: Cotton and wooden shaft swabs are NOT acceptable.
  • RNA MUST be extracted within 12 hours after sample collection.

Storage & Stability
(Before Processing)

  • Storage: Ref (2°C to 8°C)
    Stability: 12 hours

Transport Temperature:
Ref (2°C – 8°C)

Test Category:
Routine

Test Schedule:
RNA extraction:
Monday – Friday (cut-off: 1200H)
Running day:
Monday – Friday (cut-off: 0800H)

Results Availability:
Within 24Hrs:
Specimen received before 0800H (Monday to Friday)
Next day:
Specimen received 0801H onwards (Monday to Thursday)
Monday:
Specimen received 0801H onwards (Friday)

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